Identification of Newly Isolated Xanthomonades by Using Pathological, Chemical and Physiological Tests

ISSN: 0973-7510

E-ISSN: 2581-690X

Khalid A. Ali AbdelRahim^1,2 , M.M. Ibrahim^1,3 and Gehan A. El-Gaaly⁴

¹Department of Botany and Microbiology, College of Science, King Saud University P.O. Box 2455, Riyadh 11451, Saudi Arabia.

²Department of Botany, Faculty of Science, Sohag University, 82524 Sohag, Egypt.

³Department of Botany and Microbiology, Faculty of Science, Alexandria University, P.O. Box 21511, Alexandria, Egypt.

⁴Department of Botany and Microbiology, Female Section, College of Science, King Saud University, P.O. Box 22452, Riyadh, 11495, Saudi Arabia.

J Pure Appl Microbiol. 2013;7(Spl. Edn.: November):149-158

Received: 06/08/2013 | Accepted: 19/10/2013 | Published: 30/11/2013

Abstract

Thirty newly isolated Xanthomonas-strains from different host were characterized by pathological, chemical and physiological tests.All strains were Gram-negative with short rods. Scanning electron microscopy showed that the newly isolated strains are polar-monoflagellated short rods.The bacteria were oxidase-negative, utilized glucose oxidatively and not fermentatively. All strains were able to hydrolyse starch and esculin. All of the strains tested grew on 0.02% TTC and were unable to grow on 0.1% TTC. Some of newly tested strains induced a typical hypersensitive reaction (HR) in tobacco leaves, but all the strains tested induced a typical HR in tomato leaves. Therefore, it is suggested to use tomato leaves instead of tobacco leaves for testing the HR inducing capability of Xanthomonas strains. The Rfvalues of TLC test and UV spectrophotometric analysis of newly tested strains pigments (xanthomonadin) were similar to those used as reference strains.

Keywords

Xanthomonads, Xanthomonadin, Chemotaxonomic, HR test

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