ISSN: 0973-7510
E-ISSN: 2581-690X
Lactobacilli are major part of microflora of the human gut and of many fermented dairy products and are found in variety of environments. A total of 30 strains of probiotic lactobacilli (previously isolated from milk and curd samples) were tentatively identified as Lactobacillus on the basis of their cultural, morphological and biochemical characteristics. Among the 30 isolates, five isolates namely CM25, CM27, CM28, CM33 and CM34 were identified as Lactobacillus when subjected to genus specific PCR. Species level identification of isolates was done using PIB Bryant software. Isolates CM25 was identified as Lactobacillus plantarum, CM27 as L. lactics, CM28 as L. fermentum, CM33 as L. casei subsp. rhamnosus and CM34 as L. casei subsp.casei.
Lactobacillus, PCR, PIB software
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