ISSN: 0973-7510
E-ISSN: 2581-690X
Identification of dermatomycetes with the use of traditional methods is often problematic. This is due to microscopic or macroscopic atypical morphological features that occur when cultured on nutrient media. The purpose of this study was to evaluate the possibility of using multilocus sequence typing using primers ITS region (ITS4/ITS5) and SSU region (NS1/NS4), RPB1, RPB2 to identify dermatomycoses pathogens. Regions of DNA dermatomycetes were amplified by PCR and it was sequenced. Nucleotide sequences were analyzed and combined in a common sequence which was identified in the GeneBank by BLAST algorithm. According to fungi identification except classical dermatomycoses pathogens, they are mold and yeast.
Multilocus sequence typing, Dermatomycetes, Identification, Primers, ITS region
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