ISSN: 0973-7510
E-ISSN: 2581-690X
One of the main virulence factors of S.aureus is a superantigene, named enterotoxin type E. The entE gene (693 bp) was isolated from the native strains of S. aureus. It was amplified with PCR and sequenced. The entE gene was cloned into a pET-28a expression vector. Then, the recombinant plasmids were electroporatively transformed into E. coli Rosetta BL-21(DE3) strain and was expressed. The recombinant entE was purified and confirmatory tests were performed. The results showed the specific pattern and it¢s similarity with the reference entE gene. The recombinant protein (~30Kda) was confirmed by western blotting. RFLP pattern, sequencing and western blotting of the gene product confirmed the entE gene. Therefore, the method of cloning and expression of entE gene was set up and paving the way for the production of specific antibody and achievement of rapid diagnostic method for SEE.
Staphylococcus aureus, Recombinant protein. Enterotoxin E. Cloning. Expression
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