In the present study, we have investigated the ability of bacterial antagonist, Bacillus megaterium strain EXB-53 to promote plant growth in different vegetable crops (chilli, capsicum, egg plant, tomato, cabbage and cauliflower) in commercial nursery. Our results suggested that seed treatment with talc based formulation of B. megaterium strain EXB-53 significantly increased vigour index up to 245% in different vegetable crops over untreated control plants. In addition, fresh plant weight increased up to 121% and dry weight up to 70%. Furthermore, the strain showed extensive root colonization ability and positive to multiple plant growth promoting traits such as indole-3-acetic acid (IAA) production, phosphate solubilization and production of volatile compounds. The fermentation process of EXB-53 was successfully scaled-up to 10L using a molasses urea medium and higher biomass (14.41Log₁₀cfu ml^-1) was obtained at the end of 72hr after inoculation. Hence, in the current investigation, the possibility of exploiting B. megaterium EXB-53 for development of commercial plant growth promoting product of vegetable crops is discussed.