Monokaryons are fundamental materials for genetics and breeding research in Lentinula edodes. In this study, growth rate (GR), biomass (BM), germination period (GP) and mating type (MT) of 157 sporulated monokaryons from shiitake hybrid L6-26 were determined, and genetic analysis was then carried out to reveal the genetic correlation of these traits. Results showed that GR, BM and GP appeared continuous variation, which suggested these traits were controlled by polygenic system. Broad-sense heritability of GR and BM were 97.9% and 89.1% respectively, which demonstrated that GR and BM were significantly affected by genotype. The coefficients of correlation between GR, BM and GP indicated that there was significantly positive correlation between GR and BM, while GP were negatively correlated with GR and BM. Results of one-way analysis of variance (ANOVA) also showed genetic correlation between GR and MT in L. edodes monokaryons. Further results demonstrated that MT could explain 9.0% of total GR variation. To our knowledge, it was the first study on the genetic relationship between GR and MT in shiitake mushroom.