ISSN: 0973-7510
E-ISSN: 2581-690X
Polyphenol oxidase plays a key role in plant defense systems. Screening of tobacco (Nicotiana tobacum) leaves grown in Northern Iran demonstrated that there was a polyphenol oxidase with some similarities with polyphenol oxidases from other sources. Using the fresh leaves of the plant as a source, the polyphenol oxidase content was isolated, purified and characterized. The specific activity of polyphenol oxidase was increased at various stages of purification process. Its optimum pH and temperature were similar to those reported for polyphenol oxidase from citrus grown in other parts of the world.
Polyphenol oxidase, Nicotiana tobacum, tobacco, enzyme extraction, purification
© The Author(s) 2008. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.