ISSN: 0973-7510

E-ISSN: 2581-690X

Open Access
Jesteena Johney1 , S. Radhai Sri2 and R. Ragunathan3
1Department of Food and Nutrition, Research and Development Centre, Bharathiar University, Coimbatore – 46, Tamilnadu, India.
2Department of Nutrition and Dietetics, PSG College of Arts and Science, Coimbatore -14, Tamilnadu, India.
3Centre for Bioscience and Nanoscience Research, Eachanari, Coimbatore – 21, Tamilnadu, India.
J Pure Appl Microbiol. 2018;12(3):1631-1640
https://doi.org/10.22207/JPAM.12.3.70 | © The Author(s). 2018
Received: 10/06/2018 | Accepted: 20/07/2018 | Published: 30/09/2018
Abstract

Biopolymer of chitin and chitosan were extracted from the two Pleurotus spp. of P.floridanus and P.djamor. After the optimization, genomic DNA was isolated, and by using ITS 1 and ITS 4 primers molecular identification was carried out and the nucleotides were submitted to NCBI. The biopolymer have a great commercial importance now a days because of it high efficiency in all the field. The present study was focused on to extract the chitin and chitosan from the Pleurotus spp. and these polymer was  subjected to analysis the total antioxidant activity, antimicrobial activity against food borne pathogens of E.coli, B. cereus, S.aureus, A.niger and A.flavus. Cytotoxicity study against HeLa Cell Line was also performed to study the efficacy of the extracted polymer.

Keywords

Pleurotus spp, chitin, chitosan, total antioxidant, antimicrobial activity, Cytotoxicity

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