Often, housewives are injured in the hands, which may expose them to infection, possibly with different diseases, when they are exposed as is the case in this study, which was dependent for isolation, identification of Enterobacter cloacae from different healthy fishes and experimental infection for bacteria in mice. The thirty clinical specimens were collected from fishes is diagnosed according cultural and biochemical tests as well as the central health laboratories/enterobacterial unit/Bagdad. The Murine Model of Wound E. cloacae Infection were done with 2×10⁵ ID₅₀ CFU/ml and the bacteria was isolated from infected mice group to different organs with high prevalence result of 100% percent from intestine, duodenum and blood, the spleen and liver give percentage 83% from bacteria with smallest prevalence from heart 66% and brain 50% respectively, the pathogenicity will be study and all infected group give positive results.