The aim of this study was to isolate aflatoxigenic Aspergillus flavus from ten sample pasta in Riyadh (Saud Arabia) and evaluation of various solvent extracts of Eucalyptus globulus leaves on aflatoxin production. Six isolates (No. A2, A3, A6, A7, A9 and A10) of Aspergillus flavus expressed as positive for aflatoxin production while  four isolates Aspergillus flavus expressed as negative  by detection of aflatoxigenic isolates under UV radiation, whereas isolates no A6 was gave a negative result when estimating the aflatoxin by HPLC. The antiaflatoxin of Eucalyptus (Eucalyptus globulus) were extracts by three various solvents (Acetone – Ethanol – Methanol). Methanol and ethanol extract of Eucalyptus globules were more responsible for decrease aflatoxin production followed by acetone extract. In this study, Extraction by methanol was the best in separation many component by GC-MS followed by ethanol while extraction by acetone were recorded very few compounds. Photochemical analysis (Tannins, Glycosides, Reducing sugars, Anthraquinones, Phenolics and Alkaloids) of the leaves of Eucalyptus globulus using various solvents (acetone, ethanol and methanol) have been described in previous studies.