Cancer is life threatening disease which decrease the quality of the human life and the available medicine to treat the cancer has adverse side effects. The current investigation is to uncover natural products to treat cancer without any side effects. Punica granatum L. (Pomegranate) belongs to the family fruit-bearing deciduous small plants with unique flavour, taste, nutritionally rich and popular in health promoting characteristics. P. granatum L peels were collected and extracted for phytochemicals analysis using solvent ethanol and the preliminary analysis revealed the presence of phytochemicals. The analytical GC-MS was carried out to confirm the phytochemicals with pharmacologically and biologically active compounds. The anticancer activity of the extract was carried out against oral cancer cell line (KB 3-1) and this peel extract exhibited promising anticancer property. The MTT assay showed 94.53% inhibition on the oral cancer cell line and clonogenic assay showed decrease in the colonies after treating with the peel extract. These showed positive and encouraging results with efficient anticancer properties and further studies can help identify novel compounds with pharmacological properties for effective cancer treatment.