Pseudomonas aeruginosa is the leader among gram-negative bacteria in causing burn wound infections. Exotoxin A (ETA) is a major virulence factor that produced by this organism. Specifically infecting strains tend to elaborate a smooth lipopolysaccharide (LPS) and they are resistant to the bactericidal effects of serum. It is important that antitoxin A antibody and anti-LPS antibody may provide protection through an independent and additive mechanisms.Howeverappears that optimal protection againstP. aeruginosawould be obtained by use of a vaccine capable of engendering both anti-LPS and antitoxin A antibodies. P.aeruginosa is motile with a single polar flagellum that has played an important role in the pathogenesis. Promising results in researchs show prevention of the acquisition of P. aeruginosa infection in CF patients immunized with a bivalent type a and b flagellum vaccines. One of the most component of colonization is the adhesion of type IV pili to asialo-GM1 receptors on the surface of epithelial cells. The toxin-pilin protein can candidate vaccine to prevent Pseudomonas colonization in CF.P. aeruginosa has an outer membrane which contains Protein F (OprF). The outer membrane protein F gene (oprF) of Pseudomonas aeruginosa was recently showed protect in mice against P. aeruginosa chronic pulmonary infection. P. aeruginosa is an opportunist pathogen that causes acute life-threatening infections, including pneumonia and bacteremia, individuals with immunocompromised. The control of infectious diseases requires multiple approaches and vaccination is an extremely attractive way that can induce long-term protection.