Evaluation of Green Synthesis of Ag Nanoparticles using Eruca sativa and Spinacia oleracea Leaf Extracts and their Antimicrobial Activity

ISSN: 0973-7510

E-ISSN: 2581-690X

Evaluation of Green Synthesis of Ag Nanoparticles using Eruca sativa and Spinacia oleracea Leaf Extracts and their Antimicrobial Activity

Ibrahim A. Alaraidh¹, Mohamed M. Ibrahim^{1, 2} and Gehan A. El-Gaaly³

¹Botany and Microbiology Department, Science College, King Saud University, 11451-P.O. Box 2455, Riyadh, KSA.

²Botany and Microbiology department, Faculty of Science, Alexandria University, P. O. Box 21511, Alexandria, Egypt.

³Botany and Microbiology Department, Science College (Female Center for Scientific and Medical College), King Saud University, 11495-P.O. Box 22452, Riyadh, KSA.

J Pure Appl Microbiol. 2013;7(Spl. Edn.: November):277-284

Received: 29/08/2013 | Accepted: 02/11/2013 | Published: 30/11/2013

Abstract

Eruca sativa and Spinacia oleracea plants were used to evaluate their extra cellular potential synthesis of silver nanoparticles and their bactericidal impact on Streptococcus pneumoniae and Pseudomonas aeruginosa. Aqueous solutions of AgNO₃are mixed with plant extracts. Transmission electron microscopy (TEM) was used to characterize the morphology of the nanoparticles obtained from plant extracts. Energy dispersive X-ray (EDX) spectrometer established the existence of elemental sign of the silver and homogenous allocation of silver nanoparticles. Diffraction by using X ray (XRD) analysis for the formed AgNPs revealed spherical plus cubical shapes structure with different planes ranged between 111 to 311 planes. Scanning electron microscopy (SEM) was used to characterize the morphology of the nanoparticles obtained from plant extracts. The antibacterial action of AgNPs against human pathogens, Streptococcus pneumoniae and Pseudomonas aeruginosa was recognized. Our work showed a rapid, eco-safety and suitable method for the synthesis of AgNPs from Eruca sativa and Spinacia oleracea leaf extract and can be used in pharmaceutical and other biomedical applications.

Keywords

Nanoparticles, TEM, EDX, Green biotechnology, Eruca, Spinacia

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