ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Chau Thanh Truc, Pham Thi Thu Ha , Nguyen Thi Ngoc Tram and Do Thi Duyen
Faculty of Applied Sciences, Ton Duc Thang University, Ho Chi Minh City, Vietnam.
J Pure Appl Microbiol, 2019, 13 (3): 1389-1396 | Article Number: 5623
https://doi.org/10.22207/JPAM.13.3.09 | © The Author(s). 2019
Received: 10/04/2019 | Accepted: 08/08/2019 | Published: 03/09/2019
Abstract

Rice blast is a dangerous disease that causes major damage to rice productivity worldwide. The purpose of this report is to examine the protease activity of Pyricularia grisea and the effects of Commelina communis L. (common name “day flower”) extract on mycelial growth. The result showed that three isolates were collected from wild rice (Oryza rufipogon) based on a designation of new international standard and namely as U43-i4-k024-z05-ta532 (isolate 1); U12-i0-k101-z05-ta102 (isolate 2) and U43-i4-k141-z15-ta522 (isolate 3). Particularly, the third isolate showed the highest protease enzyme activity of 30.11 U/ml, followed by isolate 1 (20.27 U/ml) and isolate 2 (12.81 U/ml). The new fungi isolated from wild rice may be important methods to control detrimental diseases infesting cultivated rice. The result showed methanol extract from the day flower inhibitions on P.grisea. Besides protease activity, fungi inhibition was also affected. The protease activity was low; the mycelial growth inhibition was high (IC50 = 2.35mg/mL at Isolate 2). Plant extracts from the day flower saved money and were environmentally friendly. So the implementation of this substance is very essential.

Keywords

protease activity, Pyricularia grisea, crude extract, antifungal activity, mycelial growth.

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© The Author(s) 2019. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.