Plant growth promoting rhizobacteria (PGPRs) are a group of bacteria that can actively colonize plant roots and can modulate plant growth. The present study was conducted to examine the effects of bio-priming with Azotobacter and Azospriliumon  on the yield and resistance to drought stress of F. arundinacea Schreb seeds under different four levels of osmotic potential such as 0.5,1,1.5 and 2 Mpa that created using the polyethylene glycol. Seeds treated for 2 and 4 days. Drought stress on the field capacity, in the four levels of 100%, 75%, 50% and 25% of field capacity was applied during plant growth. The results showed that bio-priming treatments in the traits of root length, stem length and fresh stem weight improved yield and increased resistance to drought stress as compared to control. Both types of bacteria Azospirillum and Azotobacter significantly increased yield compared to control. The performance of Azotobacter was relatively higher than in Azospirillum. With regard to F. Arundinacea species seeds treated with Azotobacter 2 and 0.5 MPa for 2 days were identified as the superior treatments.