ISSN: 0973-7510

E-ISSN: 2581-690X

Angira Ghosh, Sourav Bhattacharya and Arijit Das
Department of Microbiology, Genohelix Biolabs, A Division of Centre for Advanced
Studies in Biosciences, Jain University 127/2, Bull Temple Road, Chamarajpet,
Bangalore – 560 019, Karnataka, India.
J Pure Appl Microbiol. 2012;6(2):837-844
© The Author(s). 2012
Received: 29/12/2011 | Accepted: 28/02/2012 | Published: 30/06/2012
Abstract

Poly- b -Hydroxybutyrate (PHB), a biodegradable polymer, is accumulated intracellularly in a variety of microorganisms under controlled concentrations of nutrients such as nitrogen, oxygen and mineral ions. In this study, the potency for PHB synthesis was tested for bacteria isolated from the rhizosphere of Azadirachta indica. Out of the 9 different isolates, RS6 was selected for further studies. Under the unoptimized condition, RS6 produced 0.20 g/L of PHB in nitrogen deficient broth. Parameters like variation of carbon and nitrogen sources, temperature, pH, incubation period revealed that the highest PHB yield was obtained with glucose (10% w/v) and peptone (1% w/v) as supplements. The optimum pH, temperature and incubation period was found to be 7.0, 35°C and 48 h respectively. Following the optimization of different cultural conditions a 3 fold increase in the PHB production was recorded. Based on the biochemical studies, the isolated strain was identified as Azotobacter vinelandii. The present study provided useful data about important media and physical parameters for PHB production that can be utilized in an industrial perspective in the near future.

Keywords

Biodegradable polymer, PHB synthesis, Azotobacter vinelandii

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© The Author(s) 2012. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.