ISSN: 0973-7510

E-ISSN: 2581-690X

Annu Goel and Leela Wati
1Department of Microbiology, CCS Haryana Agricultural University, Hisar-125 004, India.
J Pure Appl Microbiol. 2013;7(4):3213-3218
© The Author(s). 2013
Received: 18/02/2013 | Accepted: 21/04/2013 | Published: 30/12/2013
Abstract

This paper presents a study on the use of rice (Oryza sativa) straw biomass for ethanol production. Alkali treated rice straw was successfully converted to ethanol using commercial cellulase and Saccharomyces cerevisiae HAU-1 strain. Treatment of 0.5 mm straw with 2% sodium hydroxide at 15 psi for 1 h resulted in 70% lignin removal along with 88% cellulose recovery. The optimum concentration of cellulase enzyme and hydrolysis temperature was found to be 7.5 FPU/ g substrate at 50°C that resulted in about 75% sugar release from polysaccharides. Fermentation of enzymatic hydrolysate by Saccharomyces cerevisiae (HAU-1) resulted in production of 20.46 g/l ethanol after 72 h incubation at 30°C.

Keywords

Bioethanol, Rice straw, Saccharomyces cerevisiae, Fermentation

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