The present study was aimed to enumerate Staphylococcus aureus in raw chicken samples sold in Bikaner city (Rajasthan) and to study antibiotic sensitivity pattern of isolated S. aureus. For this a total 50 chicken samples were collected from various meat shops out of which 48 (96%) samples were found to be contaminated with S. aureus. S. aureus counts from all the positive samples ranged between 2.699 to 6.732 log 10 cfu/g, with average counts of 4.587 log 10 cfu/g. Out of these 48 isolates 20 (41.66%) were found coagulase positive. Antibiotic sensitivity test for the isolates revealed that all isolates of chicken meat samples (n = 48) were sensitive to ciprofloxacin and doxycycline (100%). S. aureus isolates were also found highly sensitive to gentamycin. In contrast, all isolates of S. aureus (100%) were resistant to ampicillin and cloxacillin while most of the isolates were also highly resistant to tetracycline. Multidrug resistance was also found in most of the isolates in the present study. The study revealed high prevalence of S. aureus in raw chicken meat samples and also evidenced antibiotic resistance of the organism. The study reflects the poor hygienic condition of slaughtering and handling of chicken meat.