ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Ahmed Nour El-Deen1,2 , Akram Alghamdi1 , Amal Al-Barty1 , Hadeer Darwish3,4 , Basem Samra1,5 , Saqer Alotaibi3 and Bandar Al Bogami1
1Biology Department, Faculty of Sciences, Taif University, 21974 Taif, Saudi Arabia.
2Agricultural Zoology Department, Faculty of Agriculture, Mansoura University, 35516 Mansoura, Egypt.
3Biotechnology Department, Faculty of Sciences, Taif University, 21974 Taif, Saudi Arabia.
4Medicinal and Aromatic Plants Department, Horticulture Institute, Agriculture Research Center, Egypt.
5Pomology Department, Faculty of Agriculture, Mansoura University, 35516 Mansoura, Egypt.
J Pure Appl Microbiol, 2019, 13 (3): 1499-1508 | Article Number: 5830
https://doi.org/10.22207/JPAM.13.3.21 | © The Author(s). 2019
Received: 04/08/2019 | Accepted: 10/09/2019 | Published: 24/09/2019
Abstract

Entomopathogenic bacterium, Xenorhabdus has a mutualistic relationship with entomopathogenic nematode of the genus Steinernema and produces several bio-agent compounds with antimicrobial and nematicidal activities. Root-knot nematodes are considered one of the most important pests facing the cultivation of grapevine worldwide. A micro-plot field trial was conducted in naturally infested soil with Meloidogyne incognita to evaluate the potential of two strains of entomopathogenic bacteria namely Xenorhabdus budapestensis DSM 16342 (EMA) and X. szentirmaii DSM 16338 (EMC) applied separately or integrated with neem cake and/or furadan at half of recommended dose on nematode development and growth improvement of Taify grapevine. Data of nematode populations, number of galls and egg-masses, eggs/g root, plant lengths and weights and number of leaves were recorded four months after application. Results appeared significant differences between treatments and control. The triple application was more effective than dual and single applications in reducing nematode infestation and improving plant growth. Combined application of EMC or EMA with furadan or neem cake increased the efficacy (64.6-68.6%) and improved plant fresh weight (27.4-69.5%). Conclusively, utilization of such bacterial filtrates with either neem cake and/or nematicide could gain a successful approach in integrated nematode management programs.

Keywords

Entomopathogenic bacteria, neem cake, nematicide, Meloidogyne incognita, integrated management, Grapevine.

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© The Author(s) 2019. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.