The recombinantendochitinase (rChi21702) from apsychrophilic Antarctic bacterium, Sanguibacter antarcticus(KCTC 13143, PAMC 21702),has been produced using Pichia pastorisexpression system. In this study, theoptimumfermentation temperature (20°C) and growth media pH (6) for Chi21702 production was determinedusing a2-stage fed-batch culturesystem.The maximal enzyme activity (178 U/l)of rChi21702obtained using a glycerol-methanol fed-batch culture system wasapproximately6-fold higher than that of unoptimized conditions. In addition, therChi21702 exhibitedcold-activeendochitinase activity and higher thermal stability than that of the wild-type Chi21702.This work allows forlarge-scale production ofrChi21702, which could be extended to further application studies using recombinant chtinases.
Antarctica, Cold-active, Endochitinases, Fed-batch, Pichia pastoris
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