Endophytic microorganisms mostly comprising of bacteria and fungi are a diverse group of microbes, existing within a unique biological niche which is the intracellular spaces of higher plants.  In this study, 17 endophytic fungi and 14 endophytic bacteria were isolated from the medicinal plants.  These endophytes displayed the ability to produce some important enzymes namely amylase, protease and cellulase when screened using starch, casein and CMC as substrates respectively.  The endopytic fungi Guignardia mangifera (CosA) isolated from Costus igneus was having maximum amylase activity (8.9 IU/ml) compared to others while the chief producer of protease was identified as Ne1(not yet identified) isolated from Nerium odoratum with the enzyme activity of 1.5 IU/ml.  The endophytic bacteria Bacillus cereus (Adab) was showing maximum amylase activity of 0.58 IU/ml.  The isolate Bacillus thuringiensis (Ci2b) isolated from Calophylum inophyllum was having the maximum protease activity (3.5 IU/ml) compared to the other endophytes. In an attempt to optimize the conditions for maximum production, pH of the medium has been varied to study its influence on the enzyme production.