J Pure Appl Microbiol | Research Article | Volume 12, Issue 4 | Article Number: 5331

Qing Wang, Lijun Shen, Zhenzhen Zhao, Hai Yan, Qianqian Xu, Chunhua Yin, Xiaolu Liu, Haiyang Zhang and Yang Liu

School of Chemistry and Biological Engineering, University of Science and Technology Beijing,
Beijing 100 083, P. R. China.

Corresponding Author E-mail: haiyan@ustb.edu.cn
Received: 23/10/2018| Accepted: 06/12/2018 |Published: 31/12/2018
DOI: http://dx.doi.org/10.22207/JPAM.12.4.01

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ABSTRACT

The efficient culture of Rhodopseudomonas palustris using landfill leachate was investigated. Total organic carbon (TOC) and total nitrogen (TN) in landfill leachate were 16.0 g/L and 1.1 g/L at pH 4.5, respectively, which was apparently reduced with the increase of pH. Compared with carbon or nitrogen source added, the addition of phosphorus could greatly promote the growth of R. palustris using landfill leachate as culture medium. Both the growth of R. palustris and the removals of TOC and TN were enhanced by increasing inoculation ratios (v/v) of R. palustris from 20% to 50% and the highest cell density of 2.0×109 cell/mL was obtained. The viability and integrity of R. palustris was further detected with the fluorometry method of double staining and the highest proportion of 99.4% live cells was found at 72 h. This study is very important in the efficient utilization of landfill leachate to culture R. palustris as bacterial manure.

Keywords:  Rhodopseudomonas palustris, Landfill leachate, Culture, Living cell.