In the present study the oily and resinous fractions of cone from Pinus roxburghii Sarg., was evaluated at different concentration (4000, 2000, 1000 and 500 µg/mL) against the growth of some plant and human pathogenic bacteria namely; Bacillus subtilis, Escherichia coli, Ralstonia solanacearum, Erwinia amylovora and Pectobacterium carotovorum subsp. carotovorum using the Kirby-Bauer disc diffusion susceptibility test. The resinous fraction showed the highest antibacterial activity against the growth of Erwinia amylovora with inhibition zones 35 mm, 30 mm, 30 mm, and 22.33±2.51 mm, with the concentrations of 4000 µg/mL, 2000 µg/mL, 1000 µg/mL, and 500 µg/mL, respectively, and Bacillus subtilis at the concentration of 4000 µg/mL (Inhibition zone value of 13.33±1.52 mm) and at 2000 µg/mL (12 mm). The oily fraction showed good activity against Bacillus subtilis, and Ralstonia solanacearum at the concentrations of 4000 µg/mL with Inhibition zone values of 14.33±1.15 mm, and 13±1.73 mm, respectively. The resinous fraction from cone extract of Pinus roxburghii could be useful as a natural biocide tool for controlling the growth of Erwinia amylovora.