Effect of UV and EMS Mutation on Aspergillus niger in the Production of α-amylase

ISSN: 0973-7510

E-ISSN: 2581-690X

Effect of UV and EMS Mutation on Aspergillus niger in the Production of α-amylase

C. Mahalakshmi¹, S. Miller Samson² , S. Alagendran², R. Anusha² and E. Neelamathi²

¹Department of Biotechnology, Bharath College of Science and Management, Thanjavur.

²PG Department of Biotechnology, Nehru memorial College (Autonomous), Puthanampatti – 621 007, India.

J Pure Appl Microbiol. 2009;3(2):705-708

Received: 13/07/2009 | Accepted: 10/08/2009| Published: 31/10/2009

Abstract

Production of extracellular a- amylase enzyme by a filamentous fungus, Aspergillus niger was studied in rice submerged fermentation. The potential strain was successfully mutated by ultraviolet (UV) and ethyl methane sulphonate (EMS). High level of a- amylase activity was obtained by the mutant strains UV-3 and EMS-2. These strains exhibited 1.44 and 2.19 times increased production of a- amylase respectively than the wild strain, which reveals the potential use of mutant strains in the industries.

Keywords

Aspergillus niger, a- amylase, EMS, UV, Mutation, Ascomycetous fungi

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