ISSN: 0973-7510

E-ISSN: 2581-690X

Osama Y. Althunibat1 , Haitham Qaralleh2, Sati Yassin Ahmed Al-Dalin2, Muayad Abboud3, Khaled Khleifat3, Ibrahim S. Majali4, Hammad K. H. Aldal’in2, Walid A. Rayyan5 and Ahmad Jaafraa2
1Department of Laboratory Medical Sciences, Princess Aisha Bent Al-Hussein Faculty of Nursing & Health Sciences, Al-Hussein bin Talal University, Jordan.
2Department of Medical Support, Al-karak University College, Al-Balqa’ Applied University, Jordan.
3Department of Biology, Mutah University, Karak, Mutah, 61710, Jordan.
4Al-Ghad International College for Applied Medical Sciences, Tabuk, Saudia Arabia.
5Faculty of Medicine, University of Hail, Hail, KSA.
J. Pure Appl. Microbiol., 2016, 10 (1): 367-374
© The Author(s). 2016
Received: 14/12/2015 | Accepted: 03/02/2016 | Published: 31/03/2016
Abstract

This study was designed to evaluate the antibacterial activity of Thymus capitatus and two of its major components namely thymol and carvacrol. The dried leaves were extracted using soxhlet apparatus. Thymol and carvacrol were isolated using normal phase column chromatography. The antibacterial activity of the leaves extract, thymol and carvacrol were estimated using agar disc diffusion and broth dilution methods against four bacterial strains. The leaves extract was found to show a broad spectrum of antibacterial activity against all the tested bacterial strains. Pseudomonas aeruginosa was the most sensitive bacteria. The purified compounds exhibited relatively stronger antibacterial activity than the extract. Thymol fraction exhibited stronger antibacterial activity than carvacrol. Thymol fraction exhibited MIC values ranging from 0.005 – 0.008 mg/mL while carvacrol fraction exhibited MIC values ranging from 0.007 – 0.008 mg/mL.

Keywords

Thymus capitatus, Thymol, Carvacrol, Pseudomonas aeruginosa.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.