The experiment aimed to study the effects of the number of microsafe detergent spraying periods on the microbial presence in mouth and vent of birds and their product characteristics. A total of 180 one-day old unsexual chickens (Ross-308) strain were used. The birds were randomly allocated to four treatments with 3 replicated of 15 birds each. The treatments were control (C) without microsafe spray and D3, D7 and D10 which were sprayed with microsafe detergent every 3, 7 and 10 days, respectively. Total bacteria count, coliform and E.coli bacteria at 14 and 28 days in both mouth and vent of birds were significantly (p <0.05) lower when microsafe detergent was sprayed at D3, D7 and D10 treatment as compared with control (C). E. coli O157H: 7 bacteria in mouth and vent of birds at 14 and 28 days, results were indicated high effectiveness of microsafe spraying period on the elimination of this type of bacteria. Body weight, weight gain, feed intake and feed conversation ratio at 28 days were significantly (p <0.05) higher in all number of microsafe spray periods treatments as compared with control.