This study was carried out to evaluate the effect of high hydrostatic pressure on quality changes of cultured yellow croaker (Pseudosciaena crocea) muscle during storage time at 4 °C. High hydrostaticpressure (HHP) treatments were applied at  100, 200, 300, 400, and 500 MPa for 10 and 15 min. Quality changes include pH, water activity, total volatile basic nitrogen (TVB-N), thiobarbituric acid content(TBA) and trimethylamine (TMA). Total bacterial count of P. crocea muscle’s samples were determined immediately after treatment and throughout subsequent storage at 4 °C as well. Results have shown P. crocea muscle’s pH value, TBA value content and TMA content increased significantly with the increase of treated pressure, while Aw and TVB-N content decreased as the pressure increased, and the growth of total bacterial count significantly inhibited with increasing pressure. The results showed that the pH value and Aw of P. crocea muscle treated with HPP increased and then decreased during storage; the TVB-N and TMA contents both increased (no more than 35 mg/100 g and 5 mg/100 g, respectively) and were effectively controlled on day 45 after the treatment at 500 MPa for 15 min; and the total colony count of P. crocea was 5.7 × 104 CFU/mL on day 45 after the treatment at 500 MPa for 15 min. Together these results indicate that the HHP treatment at 500 MPa for 15 min is optimal for the preservation of cultured P. crocea.