Temperature is one of the most important environmental factors for cell growth and product formation. Batch microbial mannatide fermentations by a-hemolytic Streptococcus H1S-33 at various temperatures, ranging from 28 to 39 ^oC, were studied. The lag time was shorter and the specific cell growth rate and specific mannatide production rate in the early stages of growth were higher at 30 ^oC or higher temperature. The average cell growth rate was higher and the biomass reached the maximum earlier than other temperature at 37 ^oC during fermentation 0-11 h and declined obviously after 11 h, but they remained at a higher level at 33 ^oC after 11 h. Therefore the two-phase temperature control strategy was presented. The temperature control strategy was that the temperature was 37 ^oC during 0-11 h, it was adjusted to 33 ^oC with the descending 1 ^oC an hour after 11 h. The biomass (dry cell weight) and mannatide production yield reached 9.24 g·L^-1 and 1.16 g·L^-1 at the variable temperature, respectively, which were higher than those at other temperatures.