Determination of Symbiotic Effectiveness of Rhizobium Strains Isolated from Food Legumes (Bean) Collected from Fez, Morocco

ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access

Determination of Symbiotic Effectiveness of Rhizobium Strains Isolated from Food Legumes (Bean) Collected from Fez, Morocco

Halima Berrada¹, Jamal Ibijbijen² and Kawtar Fikri-Benbrahim¹*

¹Laboratory of Microbial Biotechnology, Sciences and Technology Faculty, Sidi Mohammed Ben Abdellah University, P.O. Box 2202, Fez, Morocco.

²Environment and Soil Microbiology Unit, Faculty of Sciences, Moulay Ismail University, P.O. Box 11201 Zitoune, Meknes, Morocco.

J Pure Appl Microbiol, 2019, 13 (1): 247-255 | Article Number: 5459

https://dx.doi.org/10.22207/JPAM.13.1.26 © The Author(s). 2019

Received: 09/01/2019 | Accepted: 06/02/2019 |Published: 09/03/2019

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Abstract

This study was conducted in order to evaluate the symbiotic effectiveness of Rhizobium strains isolated from food legumes (Bean) collected from different sites in Fez city and regions (Morocco). For this purpose, 15 isolates were obtained. Seeds were inoculated with these isolates and grown in bags containing sterile soil in greenhouse under controlled plant growth conditions. 30 days after planting several measurements were carried out to assess nodulation, plant growth and N₂ fixation. Nodule number and dry weight were determined as an indication of inoculation response; shoot and Root dry weights as an indication of growth response, and N-uptake as an indication of nitrogen fixation effectiveness. The results of the experiment were statistically analyzed showed a statistically significant difference between the tested strains (P < 0.05).

Keywords

Bean, Rhizobium, N2, Fixation efficiency.

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