Fusarium solani, as the most predominant agent of mycokeratitis, may also occur as a mixed infection with HSV. Furthermore due to similarities in their clinical features,   sometimes  it leads to  misdiagnosis. The aim of this research ,was the rapid and accurate detection of F. solani as a main etiological agent or as a mixed infection in cases suspected  to HSV. Accordingly, 65 negative, and 35 positive samples for HSV were collected. LAMP technique with (TEF-1a) as the target gene , was developed for   detection of F. solani. The test was carried out in 1-h reaction at 65 °C in a heater block  . Using this method for the total, 2 cases among the negative samples were found to be positive for F. solani. All were rechecked by PCR and the results were the same. The specificity of the test was 100% and its sensitivity was one copy of genome. It showed that, both   methods were equal for this purpose. However, due to   advantages of the LAMP technique, it can be a substitute for PCR, even in low technology laboratories. Besides this can be concluded, some cases  suspected to herpeskeratitis ,are related to F. solani, which could be detected by molecular methods.