ISSN: 0973-7510

E-ISSN: 2581-690X

Sandeep Bisht1, Shivesh Sharma2 , Anchal Sood1, Vivek Kumar3 and Narendra Singh Bisht4
1Department of Microbiology, SBS Post Graduate Institute of Bio-Medical Sciences & Research, Balawala, Dehradun, India.
2Department of Applied Mechanics (Biotechnology), Motilal Nehru National Institute of Technology, Allahabad, India.
3Microbiology Section, Department of Soil and Water Research, Public Authority of Agriculture & Fish Resources, P.O. Box 21422, Safat – 13075, Kuwait.
4Department of Botany, H.N.B. Garhwal University, Pauri Campus, Pauri, India.
J Pure Appl Microbiol. 2010;4(1):47-54
© The Author(s). 2010
Received: 20/11/2009 | Accepted: 26/12/2009 | Published: 30/04/2010
Abstract

The aim of this study was to isolate native microbial consortium capable of decolorizing and degrading anaerobically treated molasses spent wash (MSW). We reported decolorization and COD reduction of MSW by native microbial consortium isolated from MSW disposal site and using Bacillus megaterium, a standard culture for comparison.  Microbial decolorization and COD reduction was found to be dependent on specific carbon (glucose) concentration. Results indicate that under optimal condition of carbon for each treatment, the maximum COD reduction (62%) and decolorization (56%) was achieved at 2.5% glucose concentration with native microbial consortium, followed by Bacillus megaterium. (49.72% and 52.92%). The concentration of MSW up to 6.25% was good for higher percentage of color removal and COD reduction, but, with increasing concentration of MSW i.e. 12.5%, 18.75% and 25%, resulted in decreased decolorization and COD reduction. Experiment on fresh or used microbial cells on fresh or used medium was also done to understand the reason of decolorization.

Keywords

Native microbial consortium, COD, Decolorization, Molasses spent wash

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