Aspergillosis (AS) infection arises in more than 50% of human immunodeficiency virus (HIV) transmits a disease to Indian patients. This study was carried out to analyze the immunophenotypic and intracellular cytokine profile of patients with HIV- AS co-infection s. Fifteen patients with HIV- AS co-infection and 15 each with AS alone and healthy individuals were studied. Immunophenotypic analysis and intracellular cytokines were measured using appropriate antibodies on a flowcytometer. Percentage of CD3+ did not differ significantly in the three groups. The ratio of CD4+: CD8+ was reversed among patients with CA and HIV- AS CD19+ and CD25+ were present on fewer cells of healthy individuals but this was not statistically significant. Significantly higher percentage of cells of patients with AS and HIV- AS were CD69 positive. Interferongamma (INF-g) and tumour necrosis factor-alpha (TNF-a) levels are significantly reduced in the CD4+ cells of patients with HIV- AS when compared with those with AS and healthy individuals. In CD8+ cells of patients with HIV- AS, levels of TNF-a are higher when compared with the other two groups. Interleukin-2 (IL-2) producing cells were not significantly different in any of the above subsets. Monocytes in individuals with HIV- AS had significantly higher interleukin-6 (IL-6) and TNF-a. T-helper cells among patients with HIV- AS have significantly lower cytokine production. T-suppressor cells and monocytes produce more TNF-a. These findings may be significant in view of recent attempts to treat HIV- AS coinfected Patients with anti-TNF therapy.