ISSN: 0973-7510
E-ISSN: 2581-690X
A new strain, Bacillus sp. DG24, isolated from crude oil polluted soils, showed high biodegradability of oil under low temperature conditions. In the present work, effect of environmental factors including, crude oil concentration, temperature, salinity, pH, and nutrient on the biodegradation of crude oil as well as the biodegradability of different alkanes by Bacillus sp. DG24 were investigated. GC-MS results showed that these oil components were mainly C10-C28 alkanes, and the biodegradability of different alkanes decreased along with the increase of molecular weight. Moreover, the results also showed that the optimal substrate concentration, temerature, pH, salinity and nutrients ratio for Bacillus sp. DG24 was 100 mg·L-1, 25°C, 7-8, 1% and 20:1:1, respectively. The biodegradability of crude oil under tested conditions ranged from 0-69.12%.
Bacillus, Crude oil, Biodegradation, Environmental factors, Alkane
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