ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Aalaa Fahim Abbas1 , Aamal Ghazi Mahdi Al-Saadi1, Anwar Kadhim Hussein2 and Azhar Omran Al-Thaheb2
1Ecology Department, College of science, University of Al-Qadisiyah, Diwaniyah 58001, Iraq.
2Biology Department, College of science, University of Babylon, Hilla 51001, Iraq.
J Pure Appl Microbiol, 2019, 13 (3): 1753-1761 | Article Number: 5561
https://doi.org/10.22207/JPAM.13.3.50 | © The Author(s). 2019
Received: 05/04/2019 | Accepted: 12/06/2019 | Published: 08/08/2019
Abstract

The respiratory colonizer K. ozaenae can cause a broad spectrum of infections, and it always misdiagnoses by phenotypic procedures. The role of capsule has not been proved in K. ozaenae yet. One hundred (100) clinical specimens were taken from patients suffering different infections. After phenotypic identification by cultural, microscopic, and biochemical tests, the suspected K. ozaenae isolates subjected to molecular identification using 16S rRNA gene. The non-capsulated K. ozaenae isolate was prepared from higher muco-viscous capsulated K. ozaenae isolate. Mice were injected intraperitoneally by capsulated and non-capsulated K. ozaenae, then bacterial burden in the spleen, liver, and blood was compared and histopathological lesions were detected in liver. Susceptibility of K. ozaenae with and without capsule to different antibiotics concentrations was tested at 600nm wavelength. Phenotypically, 3 (3%) isolates of K. ozaenae were identified, while results of 16S rRNA gene were concordant in 2/3 (2%) isolates and discordant in 1/3 (1%) isolate. Significant differences were showed between the increased log number of capsulated K. ozaenae isolate and decreased log number of non-capsulated K. ozaenae isolate that recovered from mice spleen, liver, and blood. Severe pathological lesions were observed in mice liver infected by capsulated K. ozaenae compared with non-capsulated K. ozaenae isolate. No-significant differences were found between the growth of capsulated and non-capsulated K. ozaenae isolate treated with the same antibiotic concentration. 16S rRNA are useful molecular tool to avoid misidentification of K. ozaenae. Removal of capsule decreases virulence of K. ozaenae, but not affect its sensitivity to antibiotics.

Keywords

Klebsiella ozaenae, capsule, virulence, 16S rRNA, antibiotic sensitivity.

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© The Author(s) 2019. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.