J Pure Appl Microbiol | Research Article | Volume 12, Issue 4 | Article Number: 3965

Bin Rui1*, Xiaotao Wei2, Long Chen1, Yun An3 and Han Wen1*

1School of Life Science, Anhui Agricultural University, 230026, Hefei, China.
2School of Information and Computer, Anhui Agricultural University, 230026, Hefei, China.
3School of Tea and Food Technology, Anhui Agricultural University, 230026, Hefei, China.

Corresponding Author E-mail: 1485507359@qq.com
Received: 08/07/2018| Accepted: 12/09/2018 |Published: 17/10/2018
DOI: http://dx.doi.org/10.22207/JPAM.12.4.05

SHARE

ABSTRACT

Mass spectrometry is an analytical technique for molecules detection after separating the particle motion (charged molecules, atoms and fragment ions) according to their mass-to-charge ratio, under the action of an electric field and a magnetic field. In this study, ten kinds of fragment ions of metabolites in Escherichia coli were detected by ion trap liquid mass instrument and triple quadrupole mass chromatograph. Two different detection methods were then compared, to provide a foundation for later metabolomic research. This experiment detects metabolites with multistage mass spectrometry by electrospray ion trap mass spectrometry and triple quadrupole mass spectrometry, when scanning at anion mode using Agilent SB-C18 (250mm×4.6mm×5ìm) chromatographic column and using ammonium acetate and acetonitrile as the mobile phase. The result showed that both methods can act as multistage mass spectrometry, while triple quadrupole mass spectrometry enables to produce finer ions under low collision energy. Triple quadrupole mass spectrometry is a superior method to acquire a clear peak in detecting fragment ions of metabolites.

Keywords: Ion trap mass spectrometry; triple quadrupole mass spectrometry;
metabolites; mass-to-charge ratio.