The present investigation was carried out to study the Minimum inhibitory Concentration (MIC) and Time kill curve of Pantoprazole (P), a proton pump inhibitor, a substituted benzimidazole: viz. 5-Difluoromethoxybenzimidazole-2-yl-3,4- dimethoxy-2-pyridylmethyl sulphoxide. It is used for inhibition of gastric acid secretion. Metronidazole (M) is a antibiotic with better broad spectrum activity against H. pylori  infections, a gram negative organism. Pantoprazole and Metronidazole ( PM )  a fixed dose combination  (FDC) has a wide range of susceptibility to any of these drugs individually. Chemical vector Mediated compatibility(CMVC) was obtained at the R&D center for PM and FDC of P and M in Combination of  40mg : 500mg ratio. The MIC was determined by broth micro dilution method as per guidelines of National Committee for Clinical Laboratory Standards (NCCLS).  This study was aimed at evaluating microbial efficacy of PM in comparison with Pantoprazole and Metronidazole alone. Efficacy was evaluated  on the basis of  MIC and time kill curve analysis in H. pylori  (HP-B₁, HP-B₂, HP-B₃ and HP-B₄) . In  case of HP-B₁, HP-B₂, HP-B₃ and HP-B₄ MIC were found to be 0.5 mg/l, 0.5 mg/l, 0.25 mg/l, and 0.5 mg/l for  PM respectively. In pantoprazole alone the MIC was found to be 32 mg/l, 32 mg/l, 32 mg/l and 64 mg/l respectively. For  Metronidazole alone, these values were  2 mg/l, 2 mg/l, 1 mg/l, and  2 mg/l respectively.  In all organisms under study, time-kill curve analysis demonstrated bacterial maximum killing at 4 hours. In conclusion, under in vitro analysis PM was found to have more bacterial inhibiting properties than pantoprazole and Metronidazole alone in vitro analysis.