A thermophilic sulfate reducing bacterium was isolated from corrosion product of cooling tower of an Indian petroleum refinery and characterized as Desulfotomaculum geothermicum with morphological and biochemical characters. Gene coding dissimilatory sulfite reductase (dsrAB), a key enzyme in the sulfate reduction was sequenced and cloned in an expression vector and over expressed with rDNA technology. Purified protein was identified as dissimilatory sulfite reductase having the molecular size of 97.6 kDa with MALDI – MS and discussed.
Sulfate reducing bacteria, biocorrosion, dissimilatory sulfite reductase
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