The aims of this study were to test the antibacterial activity and chemical composition of various crude extracts of ginger (Zingiber officinale Roscoe.). The extract was obtained using 50% aqueous – ethanol extraction solution to extract Zingiber officinale. The extract was prepared and evaluated for antimicrobial activity against six bacterial strains by determining minimum inhibitory concentration (MIC). The results revealed that the 50% aqueous – ethanol extract is potent in inhibiting bacterial growth of both gram-positive and gram negative bacteria. The chemical composition of Zingiber officinale was analyzed by gas chromatography/mass spectroscopy (GC/MS). Results of the present study sign the interesting assurance of designing a potentially active antibacterial agent from Zingiber officinale.