ISSN: 0973-7510

E-ISSN: 2581-690X

Sobhy M. Yakout1,2 , Sherif H. Abd-Alrahman1,3, Mounir M. Salem-Bekhit4 and A. Mostafa5
1Department of Biochemistry, College of Science, King Saud University, PO Box, 2455, Riyadh, 11451, Kingdom of Saudi Arabia.
2Hot Laboratories and Waste Management Center, Atomic Energy Authority, Abu Zaabel,
Cairo 13759, Egypt.
3Pesticides Residues and Environmental Pollution Dept., Central Agricultural Pesticide Laboratory, Agricultural Research Center, Giza 12618, Egypt.
4Department of Botany and Microbiology, Collage of Science, King Saud University, P. O. 2455, Riyadh,11451 Kingdom of Saudi Arabia.
5Department of Botany and Microbiology, Collage of Science, King Saud University, P. O. 2455, Riyadh,11451 Kingdom of Saudi Arabia.
J Pure Appl Microbiol. 2013;7(2):1373-1378
© The Author(s). 2013
Received: 02/02/2013 | Accepted: 05/03/2013 | Published: 30/06/2013
Abstract

The aims of the present study were to test the antibacterial activity and chemical composition of Trigonella foenum-graecum extract. The extract was obtained using 50% aqueous – ethanol extraction solution to extract T. foenum-graecum seeds. The extract was prepared and evaluated for antimicrobial activity against six bacterial strains by determining minimum inhibitory concentration (MIC). The results revealed that the 50% aqueous – ethanol extract is potent in inhibiting bacterial growth of both gram-positive and gram negative bacteria. The chemical composition of fenugreek was analyzed by gas chromatography/mass spectroscopy (GC/MS). The hydroxymethyl furfural, gingerone, a-curcumene, bergamotene, and gingerol were the highest abundant compounds out of total 31 compounds were identified in the fenugreek extract.

Keywords

GC-MS, Phytochemicals, Antibacterial

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