ISSN: 0973-7510
E-ISSN: 2581-690X
In this study we isolated a novel bacteriophage PA26 infecting Pseudomonas aeruginosa from a water reservoir. PA26 belongs to the Myoviridae family. The genome is a double stranded DNA of 72,321 basepair. Eighty eight predicted open reading frames were found, of which fourteen showed functional homology to known proteins. 99% of the phages were adsorbed to the host within 10 minutes after infection. The eclipse period was 10 minutes, and the latent period was 15 minutes, with aburst size of 252. Infectivity was highest at pH 7 and remained stable at temperatures between 4 to 37 oC. The phage inhibited biofilm formation by P. aeruginosa and also removed preformed biofilm.
PA26, bacteriophage, Pseudomonas aeruginosa, biofilm, phage therapy
© The Author(s) 2014. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.