Characterization of Newly Isolated Xanthomonads using 16s rDNA; ITS; rep PCR (ERIC, BOX)

ISSN: 0973-7510

E-ISSN: 2581-690X

Characterization of Newly Isolated Xanthomonads using 16s rDNA; ITS; rep PCR (ERIC, BOX)

Khalid Abdullah Ali AbdelRahim^1,2 , El-Sayed Mohamed Soltan^2,3 and Klaus Rudolph⁴

¹Botany and Microbiology Department, College of Science, King Saud University P.O. Box 2455, Riyadh 11451, Saudi Arabia.

²Botany Department, Faculty of Science, Sohag University, Sohag 82524, Egypt.

³Botany department, college of science, Al-Baha University, Saudi Arabia.

⁴Institute of Plant Pathology and Plant Protection, University of Göttingen, Grisebachstr. 6, D-37077 Göttingen, Germany.

J Pure Appl Microbiol. 2014;8(Spl. Edn. 2):321-332

Received: 02/08/2014 | Accepted: 06/09/2014 | Published: 30/11/2014

Abstract

This study aimed to characterize newly isolated xanthomonads, using different genetics fingerprinting techniques. Rep-PCR fingerprinting (ERIC and BOX), 16S-23S Intergenic Transcribed Spacer-PCR (ITS), 16S rDNA amplification, were used for Xanthomonads strains characterization. By combining the ERIC and BOX PCR data using the UPGMA analysis, all strains from Lobelia and Isotoma were represented in one related group with a similarity coefficient of more than 93%. The strains from Lobelia and Isotoma could be identified as Xanthomonas lobeliae. The HV strains from cotton should be named Xanthomonas axonopodispv. malvacearum race 20. The strains from Catharanthus should be named Xanthomonas axonopodis pv. catharanthi.

Keywords

Xanthomonas species, 16S rDNA, rep PCR

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