Characterization of Brucellaphage using RAPD and microsatellite repeat marker

ISSN: 0973-7510

E-ISSN: 2581-690X

Lata Jain¹ , Mayank Rawat¹, Vinay Kumar² and Deepti Chachra³

¹Division of Biological Standardization, Indian Veterinary Research Institute, Izatnagar – 243 122, India.

²Directorate of Medicinal and Aromatic Plant Research, Boriavi, Anand, Gujarat – 387310, India.

³Department of Veterinary Microbiology, College of Veterinary And Animal Sciences, Guru Angad Dev Veterinary and Animal Science University, Ludhiana (Punjab)-India.

J Pure Appl Microbiol. 2015;9(1):683-690

Received: 20/12/2014 | Accepted: 16/01/2015 | Published: 31/03/2015

Abstract

Brucellosis is an important zoonotic infection of worldwide significance. In this study the recently isolated lytic brucellaphage ‘FLd’ against Brucella abortus was characterized using random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) markers. Out of 18 RAPD primers used, 15 primers were able to generate distinct finger prints / amplicons ranged from two to twelve fragments. The RAPD finger prints produced were distinct and highly reproducible. A total of 18 microsatellite repeat (ISSR) markers used in the study had revealed that the phage genome consists of di-nucleotide repeats of AG, TG and CT and tri-nucleotide repeat of AGC and ACG. The RAPD and microsatellite fingerprinting has generated the basic information about the genomic composition of the Indian isolate of Brucellaphage which could be useful in the identification and discrimination of new phage and high resolution brucellaphage diversity studies.

Keywords

Brucellaphage, Random amplified polymorphic DNA, ISSR marker

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