Bacteria attack all agricultural crops which are considered a source of human food in all regions of the world and causes many economic loses. Erwinia carotovora subsp attack vegetables and specially those with meat tissues such as potato tuber and cause soft Rot disease. Soft Rot in all regions of the world and especially in hot regions is one of the serious diseases that attack crops in the field, during storage and transport leading to great loss in production more than any other bacterial disease. Diseased samples of three brands of potato tubers were collected: Hermes, Lady Rosetta and Carege in addition to soil samples were also taken from the rhizosphere region surrounding the tubers. Chemo-biological tests and bacterial development on Crystal Violet Pectate (C.V.P) medium and enzymatic interaction system API 20 E stripe for identification of the bacteria associated with the family Enterobactericeae were also carried. Pure colonies of Erwinia were obtained as well as pure single colonies. The result of identification showed the presence of two types of bacteria, Erwinia carotovora (E.c) and Erwinia chrysanthemi (E.chr).