ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Anju Majeed1,2* , Muhammed Majeed1, Nooruddin Thajuddin2, Sivakumar Arumugam1, Furqan Ali1 and Kirankumar Beede1
1Sami Labs Limited, 19/1 & 19/2, 1st Main, 2nd Phase, Peenya Industrial Area, Bangalore – 560 058, Karnataka, India.
2Department of Microbiology, Bharathidasan University, Tiruchirappalli – 620 024, Tamil Nadu, India.
J Pure Appl Microbiol, 2019, 13 (2): 1159-1168 | Article Number: 5636
Received: 10/04/2019 | Accepted: 20/05/2019 | Published: 30/06/2019
Abstract

Calebin-A, a novel natural product of turmeric (Curcuma longa) rhizome has been reported to possess various pharmacological activities. The minimal natural occurrence has been the major constraint for exploiting its pharmacological use. Thus, this study focuses to investigate the production of calebin-A through biotransformation approach using curcumin as the substrate. Four bacterial strains (Acinetobacter calcoaceticus delfa L360, Pseudomonas putida TGPNP13, P.putida NCIMB 10007 and A.johnsonii NCIMB 9871) and three different media compositions (MTSB, modified tryptic soy broth; TSB, tryptic soy broth; NB, nutrient broth) were used to investigate the conversion of curcumin to calebin-A. Biotransformation studies were carried out by supplementing curcumin (50 mg) into media (1000 ml). After 24 h of interval, samples were withdrawn and ethyl acetate extract was obtained as per partition method followed by determination of calebin-A presence using HP-TLC and HPLC techniques. P. putida NCIMB 10007 and A. johnsonii NCIMB 9871 were found to convert curcumin to calebin-A in a time dependent manner with optimum conversion at 48 h and after 96 h respectively. The highest growth of P. putida NCIMB 10007 and A. johnsonii NCIMB 9871 and the production of calebin-A were observed when grown in MTSB media. Baeyer-Villiger monoxygenase enzyme was produced by P. putida NCIMB 10007 (3.30±0.09 U/ml) and A. johnsonii NCIMB 9871 (4.10±0.10 U/ml), suggesting its possible role in the conversion of curcumin to calebin-A. The findings of this study clearly suggested that P. putida NCIMB 10007 and A. johnsonii could be potential candidates for biotechnological production of calebin-A from curcumin.

Keywords

Calebin-A, biotransformation, Baeyer-Villiger monooxygenase, Acinetobacter calcoaceticus, Pseudomonas putida.

Article Metrics

Article View: 3945

Share This Article

Journal Tools

Journal Metrics 2018

© The Author(s) 2019. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.