ISSN: 0973-7510
E-ISSN: 2581-690X
The present study deals with an ecofriendly method for synthesising Silver Nano Particles (SNPs) from the supernatant of three selective test strains namely Escherichia coli, Bacillus sp and Lactobacillus sp. The synthesis of SNPs were confirmed by UV-VIS spectroscopy which revealed Surface Plasmon Resonance (SPR) absorption band located at 400nm for Bacillus sp and 420nm for both E.coli and Lactobacillus sp. The antibacterial properties of SNPs were evaluated against Gram positive Staphylococcus aureus and Gram negative Pseudomonas aeruginosa with control plates. The SNPs of Bacillus at 5µl and 10µl concentration showed considerable inhibitory zone against P.aeruginosa and Staph. aureus followed by SNPs of E.coli and Lactobacillus sp. The application studies also carried out to emphasize the bactericidal activities of SNPs on cotton textiles towards Gram negative organisms E.coli and P.aeruginosa and they revealed zone of clearance.
SNP, SPR, UV-VIS Spectrum, MHA plates, Zone of inhibition
© The Author(s) 2014. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.