In the current study, Streptomyces bikiniensis was isolated from insect (Tapinoma simrothi), Riyadh, Saudi Arabia, and identified by classical and molecular methods. The biosynthesized silver nanoparticles (AgNPs) were characterized by determining Fourier transform infrared spectroscopy (FTIR), Energy Dispersive Spectroscopy (EDS) and transmission electron microscopy (TEM). TEM photos of nanoparticles showed the spherical shapes of nanoparticles in the size range of 3–70 nm. Moreover, the minimum inhibitory concentration of biosynthesized silver nanoparticles against Staphylococcus aureus, Streptococcus pyogenes, Klebsiella pneumonia, Pseudomonas aeruginosa and Salmonella typhi were at 8 µg per paper disc (6 mm), and the inhibition zones were 7 to 9 mm while was at 16µg per paper disc in case of Candida albicans, the inhibition zone was 8 mm. The lethal inhibitory concentration (IC₅₀) of AgNPs on Hep2 and MCF-7 cells was obtained at 253.6 and 119.6 µg/ml, respectively. Thus, bioconversion of silver nanoparticles by Streptomyces bikiniensis strain Ess_amA-1 can be employed as a potential Nano-drug to kill pathogenic microbes.