This communication describes the in vitro anti-bacterial, anti-fungal and anti-oxidative evaluation results of the different extracts of Gazania (Asteraceae).  Whole plant was extracted using distilled n-hexane, DCM, ethyl acetate methanol and methanol: water subsequently. These fractions were further tested for in vitro anti-bacterial, anti-fungal and anti-oxidative activities using standard methods like micro broth dilution method for anti-bacterial and fungal, DPPH radical scavenging and H2O2 scavenging activity methods for anti-oxidative activity. The investigations resulted fractions with good anti-bacterial, anti-fungal and anti-oxidative activities, results were tabulated. Based on the results it was concluded that DCM, ethyl acetate, methanol and methanol: water fractions have good bio activity, these fractions may be further investigated for the pure compounds.