Five bacterial species namely: Brahmella catarrhalis, Proteus vulgaris, Bacillus cereus, Staphylococcus aureus and Pseudomonas aeruginosa were isolated from housefly (Musca domestica). At different populations, the active bacterial cells and their culture supernatants solutions. All the isolates expressed varying degrees of mortality. The mortality rate increased alongside increase in the bacterial cell load, concentration of supernatant and period of exposure of the larvae. Comparatively, in both active cells and supernatants, P. aeruginosa, Staph. aureus and B. cereus showed higher larvicidal activities than other bacteria. Pseudomonas aeruginosa displayed the highest larvae mortality than Staphylococcus aureus and Bacillus cereus.