The physico-chemical and Microbiological assessment in water samples of three ponds of village Irongmara, Cachar district, Assam, was carried out during pre and monsoon period (March, 2010 and May, 2010) which results decrease in the quality of water. The P^H, Total alkalinity and chloride contents ^of water samples was within limit as prescribed by WHO. Certain nutrients like nitrate and phosphate remained above the minimum level. The bacteriological investigation showed the presence of E. coli and other potentially pathogenic bacteria which may cause severe water borne diseases. The heterotrophic bacteria isolated from three ponds included Pseudomonas, Proteus, E. coli, Citrobacter and Bacillus. The observations have indicated that the water of these ponds is not safe for drinking without treatment.