ISSN: 0973-7510

E-ISSN: 2581-690X

A. Brinda Devi, C. Valli Nachiyar and T. Kaviyarasi
1Department of Biotechnology, Sathyabama University, Tamil Nadu, Chennai, India.
J Pure Appl Microbiol. 2014;8(5):4143-4147
© The Author(s). 2014
Received: 24/04/2014 | Accepted: 27/06/2014 | Published: 31/10/2014
Abstract

Bacillus cereus isolated from cloth used to smear oil on ‘dosa’ pan has been used for the production of PHB.  The influence of medium components on the production of PHB was studied using Plackett Burman screening design which indicated that glucose concentration followed by yeast extract concentration play an important role in the production of PHB.  The organism required glucose as carbon source at 0.5% and yeast extract as nitrogen source at 0.2% for the maximum production of PHB.  The PHB production by Bacillus cereus increased from 34.9% to 52.4% (1.2 g of PHB/L) after optimization.

Keywords

Polyhydroxybutyrate, Bacillus cereus, PBSD, Optimization

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